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BioTool

Experimental design

12 resources · Design primers, plan cloning and editing, and calculate experimental quantities.

Primer-BLAST

Design primers and check their specificity.
Primer design
Online tool

Primer3Plus

Design PCR primers.
Primer design
Online tool

IDT OligoAnalyzer

Analyze oligonucleotide melting temperatures, hairpins and dimers.
Primer design
Online tool

NEBuilder

Design DNA fragment assemblies.
Cloning & editing
Online tool

CHOPCHOP

Design CRISPR guide sequences.
Cloning & editing
Online tool

SnapGene

View plasmid maps and sequences, and simulate cloning.
Cloning & editing
Desktop software

NEBioCalculator

Convert nucleic acid mass, molar amounts and concentrations.
Lab calculations
Online tool

CRISPOR

Evaluate CRISPR guide sequences and potential off-target effects.
Genome editing design
Online tool

CRISPRdirect

Select CRISPR target sequences and check specificity.
Genome editing design
Online tool

NEBcutter

Find restriction enzyme sites and generate digestion maps.
Restriction analysis
Online tool

NC3Rs Experimental Design Assistant

Plan experimental diagrams, randomization, blinding and sample sizes.
Experimental design
Online tool

G*Power

Calculate statistical power and sample sizes for experimental designs.
Power & sample size
Desktop software